alpha Actinin

IgG

Chicken

alpha Actinin

The antiserum has been treated to remove lipoproteins and is supplied as a delipidized whole antiserum solution. Storage

IF: Use at a dilution of 1/500. WB: Use at an assay dependent dilution. Predicted

Cytoplasmic Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100 000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID (By similarity).

In vertebrates 3 main groups of actin isoforms alpha beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.