Myosin IIB

IgG

Human

MDCK NRK or Jurkat whole cell lysate

1 mg/ml Storage

WB: Use at a dilution of 1/200. Detects a band of approximately 200 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Myosins belong to a superfamily of actin based motor proteins comprising to date at least 15 classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin IIB (nonmuscle) is a relatively abundant widespread two headed myosin composed of an N-terminal motor domain a light chain binding neck region a coiled coil region and a nonhelical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. The vertebrate myosin II class members include muscle (sarcomeric and smooth) and nonmuscle (cytoplasmic) myosins. The non muscle myosin II class contains two isoforms: IIA and IIB which exhibit 85% and 72% amino acid identity in the motor domain and the rod respectively. Varying ratios of the two isoforms are expressed in different cells and tissues. Some cells express only a single

Muscle contraction.

Cytoplasm myofibril. Note=Thick filaments of the myofibrils.

Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Contains 1 myosin head-like domain.