GJA1

IgG

Mouse brain tissue lysate

Cytoplasmic and Integral membrane protein

15mM Sodium Azide; Constituents: 1% BSA 0. 01M PBS. pH 7. 4

Cytoplasmic and Integral membrane protein Gap junctions are aggregations of intercellular channels which directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells synchronization of cellular physiological activities growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions nucleotides and small molecules between adjacent cells. Unlike other membrane channels intercellular channels span two plasma membranes and require the contribution of hemi-channels called connexons from both participating cells. These channels accommodate molecules as large as 1 kDa. They have been detected in virtually every cell type in mammals except mature skeletal muscle spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins which have been termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights between 26 and 70 kDa. At least a dozen distinct connexin genes have been identified; many of them expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group consists of Cx26 Cx30 Cx31 Cx31. 1 and Cx32. The other termed class II or alpha group is represented by Cx33 Cx37 Cx40 Cx43 and Cx46. All of the connexins share a common membrane topology but differ in their conductance and channel gating properties. The structure of connexin molecules includes a cytoplasmic N-terminal region four transmembrane domains two extracellular loops and a C-terminal cytoplasmic tail of varying length. The various connexins are highly conserved in the transmembrane and extracellular regions but they differ in their cytoplasmic domain. The 43 kDa connexin protein (connexin 43 Cx43) belongs to the alpha type (group II) subfamily of connexin proteins. It is expressed in most tissues even though the pattern of expression may differ in various cell types. For example in the brain it is found in astrocytes ependyma and leptomeninges but not in neurons oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes. Gap junction protein levels change in response to disruption of tissue architecture. For instance an increased expression of Cx43 was found in early stages of atherosclerosis. Changes in the levels or types of connexin expressed in a given cell type have been found to correlate with tumor progression and metastasis. However glioma cells transfected with the oncogene neu (c-erb-B2) have been shown to exhibit a major reduction in intercellular communication with no decrease in overall expression of Cx43. Monoclonal antibodies reacting specifically with Cx43 may be applied in diverse cellular and molecular approaches to study gap junctions and their properties. They can also be used to correlate their expression pattern with physiological functions or pathological conditions.

A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150.

Expressed in the heart and fetal cochlea.

Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.

Belongs to the connexin family. Alpha-type (group II) subfamily.