HAT1

GWB-375F8F

Synthetic peptide: EESFQELVEDYRRVIERLAQE with N-terminal added lysine conjugated to KLH corresponding to amino acids 399-419 of Human HAT1. The corresponding sequence differs by three amino acids in chicken and by one amino acid in mouse (putative sequence).

This product specifically recognizes human HAT1.

HAT1

0. 8 mg/ml

15mM Sodium Azide; Constituents: 1% BSA 0. 01M PBS. pH 7. 4

Nuclear in S-phase cells and cytoplasmic The basic repeating unit of eukaryotic chromatin is the nucleosome which consists of 147 bp of DNA wrapped around an octameric protein core formed of two molecules each of histones H2A H2B H3 and H4. These core histones are required for nuclear packaging and DNA organization as well as for regulating various cell processes. Acetylation is the most extensively studied posttranslational histone modification. Histone acetyltransferases (HATs) are members of a superfamily of enzymes that transfer the acetyl moiety from acetyl-coenzyme A cofactor onto one or more epsilon-amino groups of lysines contained in the extended N-terminal tail domains of core histone proteins. Type A HATs are located in the nucleus and many of them play a role as transcriptional coactivators. Type B HATs traditionally thought to locate to the cytoplasm acetylate nascent cytoplasmic histones prior to chromatin assembly. HAT1 a type B HAT has been identified in several species ranging from yeast and maize to human. Native human HAT1 is a ~100 kDa heterodimer consisting of a catalytic subunit (46 kDa) and a corehistone- binding subunit (Rbap46/Hat2) that greatly stimulates the acetyltransferase activity of the holoenzyme. Human HAT1 rapidly diacetylates soluble non-nucleosomal histone H4 at lysine-5 and lysine-12 with similar efficiency. It also acetylates albeit to a lesser extent histone H2A at lysine 5. These sites are distinct from those modified in transcriptional regulation. The newly synthesized acetylated histones interact with cellular proteins such as chromatin assembly factor 1 (CAF-1) to form complexes. Following deposition of the acetylated histones into chromosomes they are rapidly deaceylated. HAT1 is present in S-phase cell nuclei and in the cytoplasm. HAT1 may be involved in processes of chromatin assembly chromatin repair maintenance of specific heterochromatin acetylation pattern and telomeric silencing.

Acetyl-CoA + histone = CoA + acetylhistone.

Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.