Myelin basic protein

GWB-3015C2

Synthetic peptide: GEIHRGEAGKKR corresponding to amino acids 19-30 of Mouse Myelin Basic Protein.

Mouse Brain Tissue Extract

Cytoplasmic side of myelin

This antibody was purified by ammonium sulphate fractionation and filtration on a Sephadex G-200 column. Storage

WB: Use at a concentration of 1 - 5 µ g/ml. Detects a band of approximately 33 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination maybe as components of transcriptional complexes and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers with each of them potentially having a specialized function. Induces T-cell proliferation.

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.

Several charge isomers of MBP; C1 (the most cationic least modified and most abundant form) C2 C3 C4 C5 C6 C7 C8-A and C8-B (the least cationic form); are produced as a result of optional PTM such as phosphorylation deamidation of glutamine or asparagine arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H) C8-A(L) C8-B(H) and C8-B(L) (H) standing for higher and (L) for lower molecular weight. C3 C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging making the protein more cationic.

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Belongs to the myelin basic protein family.