Actin skeletal muscle

GWB-56CEB4

Synthetic peptide (unfortunately the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

Skeletal muscle.

Cytoplasmic

Sodium Azide; Constituents: BSA 10mM PBS. pH 7. 4

CytoplasmicProtocols from PublicationsCitation: 1: Kim JR Kee HJ Kim JY Joung H Nam KI Eom GH Choe N Kim HS Kim JC Kook H Seo SB Kook H. Enhancer of polycomb1 acts on serum response factor to regulate skeletal muscle differentiation. J Biol Chem. 2009 Jun 12; 284(24):16308-16. Epub 2009 Apr 8. PubMed PMID: 19359245; PubMed Central PMCID: PMC2713522. Experiment Name: ImmunohistochemistryExperiment Background: Cotransfection of SRF and Epc1 in C2C12 cells elevated the transcript levels of skeletal a-actin After transfection of SRF or Epc1 C2C12 cells were subjected to immunocytochemistry. Experiment Steps: 1. Prepare Longitudinal and coronal sections of hamstring muscles from either Epc1 heterozygous or wild type mice. 2. Use Diaminobutyric acid (Vector Laboratories Inc. Burlingame CA) for color development in the bright field images. 3. Skeletal a-actin was visualized with confocal laser scanning microscopy (LSM510 Carl Zeiss Jena Germany) after probing with specific primary antibody and anti-rabbit-Alexa Fluor 568 (Invitrogen). 4. Obtain Images at the higher magnification (x 800). Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID (By similarity).

In vertebrates 3 main groups of actin isoforms alpha beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.