GPR30 protein

Synthetic peptide - KLH conjugated. Epitode: C-terminal domain of human. Mouse Homology: 83% (Identity) / 94% (Similarity)Rat Homology: 94% (Identity) / 94% (Similarity)Summary: GPR30 is a transmembrane intracellular estrogen receptor (Revankar et al. 2005). Progestin inhibits growth in breast cancer cells through GPR30.
Uses: IF WB IHC (10 ug/ml) (Optimal dilution to be determined by the researcher)

Belongs to the G-protein coupled receptor 1 family [view classification]. Summary: This protein is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3 4 5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [1] Bologa C. G. Revankar C. M. Young S. M. Edwards B. S. Arterburn J. B. Kiselyov A. S. Parker M. A. Tkachenko S. E. et al. Virtual and biomolecular screening converge on a selective agonist for GPR30[2] Vivacqua A. Bonofiglio D. Recchia A. G. Musti A. M. Picard D. Ando S. and Maggiolini M. The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells[3] Kimura K. Wakamatsu A. Suzuki Y. Ota T. Nishikawa T. Yamashita R. Yamamoto J. Sekine M. Tsuritani K. Wakaguri H. et al. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes[4] Pietras R. J. Levin E. R. and Szego C. M. et al. Estrogen receptors and cell signaling[5] Filardo E. J. and Thomas P. et al. GPR30: a seven-transmembrane-spanning estrogen receptor that triggers EGF release[6] Strausberg R. L. Feingold E. A. Grouse L. H. Derge J. G. Klausner R. D. Collins F. S. Wagner L. Shenmen C. M. Schuler G. D. Altschul S. F. et al. Generation and initial analysis of more than 15 000 full-length human and mouse cDNA sequences.