Anti-Fibronectin

Fibronectin

This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5 000 to 1:10 000. \' s anti-Fibronectin detects by western blot intact fibronectin (Invitrogen Cat. No. 33016-015) after digestion by Matrix Metalloproteinase-3 (MMP-3) overnight at 37\' C. Separation was performed using a 4- 12% Tris-Glycine gel. Under these conditions a sizeable dark band at ~220 kDa representing the undigested fibronectin as well as many smaller bands representing the variably sized fragments resulting from fibronectin digestion by MMP-3 were noted. The blots were developed using the colorimetric Alkaline Phosphatase method. This product was also assayed against 1. 0 ug of Fibronectin in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H& L] (Goat) catalog # 18-732-292598 and (ABTS (2 2\' -azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4 000 to 1:8 000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher. Physical State: Liquid (sterile filtered)Purity and

1. 0 mg/ml (by A280 nm)

None

Fibronectins bind cell surfaces and various compounds including collagen fibrin heparin DNA and actin. Fibronectins are involved in cell adhesion cell motility opsonization wound healing and maintenance of cell shape.

Secreted extracellular space extracellular matrix.

Sulfated (By similarity).

Contains at least 2 fibronectin type-I domains.