IRSp53


ArtNr	18-783-313074
Hersteller	GENWAY
Menge	0.1 mg
Kategorie	Oncology Antibodies Western Blot ELISA Primary Antibodies By Organ Testicular & Prostate Cancer
Typ	Antibody
Applikationen	WB, ELISA
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-F27925
Similar products	18-783-313074
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Genway ID:	GWB-F27925
Specificity:	IRSp53 NCBI
Gene ID:	10458
Specificity:	IRSp53
Immunogen:	Peptide sequence C-HGDGSARTLAGR corresponding to the C-Terminal region of IRSp53 (NP_059345. 1).
Specificity Note:	This product specifically recognises an epitope within the C-terminal (CT) region of isoform 2 of human IRSp53 (insulin receptor substrate p53) otherwise known as BAIAP2 a brain-enriched scaffolding protein which plays a key role in actin cytoskeletal reorganization and filopodia formation acting as a signal transducing adaptor between membrane-bound Rho family GTPases Rho Rac and cdc42 and Rho family effectors mDia WAVE2 and Mena.
Antiserum Preparation:	Antiserum to human IRSp53 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution:	Phosphate buffered saline pH7. 3
Preservative Stabilisers:		0. 02% Sodium Azide (NaN3) 0. 5% Bovine Serum Albumin Suggested
Dilution:	Western Blot: This product detects a band of approximately 60kDa in human placenta cell lysates. Suggested
Dilution:	ELISA - 1:1000Western Blotting - 0. 2 - 0. 5ug/ml
Function:	Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation.
Subunit:	Homodimer. Interacts with CDC42 and RAC1 that have been activated by GTP binding. Interacts with ATN1 BAI1 EPS8 SHANK1 SHANK2 SHANK3 WASF1 and WASF2. Interacts with ENAH after recruitment of CDC42. Interacts with TIAM1 and DIAPH1 (By similarity).
Subcellular Location:	Cytoplasm. Membrane; Peripheral membrane protein. Cell projection filopodium. Cell projection ruffle. Note=Detected throughout the cytoplasm in the absence of specific binding partners. Detected in filopodia and close to membrane ruffles.
Tissue Specificity:	Isoform 1 and isoform 4 are expressed almost exclusively in brain. Isoform 4 is barely detectable in placenta prostate and testis. A short isoform is ubiquitous with the highest expression in liver prostate testis and placenta.
Domain:	The IMD domain forms a coiled coil. The isolated domain can induce actin bundling and filopodia formation. In the absence of G-proteins intramolecular interaction between the IMD and the SH3 domain gives rise to an auto-inhibited state of the protein. Interaction of the IMD with RAC1 or CDC42 leads to activation.
Domain:	The SH3 domain interacts with ATN1 BAI1 WASF1 WASF2 SHANK1 DIAPH1 and ENAH.
Ptm:	Phosphorylated on tyrosine residues by INSR in response to insulin treatment.
Similarity:	Contains 1 IMD (IRSp53/MIM homology) domain.
Similarity:	Contains 1 SH3 domain. 1. Millard. T. H. et al. (2005) Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.

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