RABBIT ANTI PCNA

ArtNr	18-783-313772
Hersteller	GENWAY
Menge	0.05 mg
Kategorie	Oncology Antibodies Western Blot Primary Antibodies By Organ Bladder Cancer
Typ	Antibody
Applikationen	WB
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-F8F3D4
Similar products	18-783-313772
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Genway ID:	GWB-F8F3D4
Specificity:	PCNA
Isotype:	Polyclonal IgGSpecies Cross Reactivity: Reacts with: Chicken Fish Rat Amphibia Bovine Monkey Mouse DogN. B. Antibody reactivity and working conditions may vary between species.
Buffer Solution:	Phosphate buffered saline pH7. 2
Preservative Stabilisers:		0. 09% Sodium Azide (NaN3)Approx. Protein Concentrations: IgG concentration 0. 5 mg/ml
Immunogen:	Synthetic peptide corresponding an internal region of human PCNA protein.
Specificity:	Recognises Human Proliferating Cell Nuclear Antigen (PCNA) a nuclear protein vital for cellular DNA synthesis. PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Recommended Secondary Antibodies: Sheep Anti Rabbit IgGGoat Anti Rabbit IgG (Fc)Goat Anti Rabbit IgG (H/L)
Function:	This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase\' s processibility during elongation of the leading strand. Ref. 28Subunit structureHomotrimer By similarity. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1 POLH POLK DNMT1 ERCC5 FEN1 CDC6 APEX2 and POLDIP2. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A By similarity. Interacts with POLD1 POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Ref. 28Ref. 10Ref. 11Ref. 12Ref. 13Ref. 14Ref. 15Ref. 16Ref. 17Ref. 18Ref. 19Ref. 20Ref. 21Ref. 22Ref. 25Ref. 26Ref. 27Ref. 30Subcellular locationNucleus. Post-translational modificationUpon methyl methanesulfonate-induced DNA damage mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through \' Lys-63\' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases HLTF RNF8 and SHPRH which is required for DNA repair. \' Lys-63\' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells and is mediated by the DCX(DTL) complex leading to enhance PCNA-dependent translesion DNA synthesis. Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Miscellaneous:	Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus. Sequence similaritiesBelongs to the PCNA family. 1. Funaro A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies. In Leucocyte Typing V: White Cell Differentiation Antigens. 2. Funaro A. et al. (1995) Human CD57 a link molecule between leucocyte and neural cells. In: Leucocyte Typing V: White Cell Differentiation Antigens. [1] \" Cloning and chromosomal mapping of human glucuronyltransferase involved in biosynthesis of the HNK-1 carbohydrate epitope. \" Mitsumoto Y. Oka S. Sakuma H. Inazawa J. Kawasaki T. Genomics 65:166-173(2000) [PubMed: 10783264] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. [2] \" Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). \" Ebert L. Schick M. Neubert P. Schatten R. Henze S. Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [3] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. [4] \" Structural basis for acceptor substrate recognition of a human glucuronyltransferase GlcAT-P an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1. \" Kakuda S. Shiba T. Ishiguro M. Tagawa H. Oka S. Kajihara Y. Kawasaki T. Wakatsuki S. Kato R. J. Biol. Chem. 279:22693-22703(2004) [PubMed: 14993226] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (1. 85 ANGSTROMS) OF 83-334.

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