AIF

E20

WB: 1:1 000IHC: 1:500ICC: 1:500FC: 1:50IP: 1:10. AIF or apoptosis-inducing factor is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death or apoptosis (1 2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation(1). Similar to cytochrome c AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c however AIF acts in a caspase-independent fashion (2 3 4). 1. Klooster JP Jansen M Yuan J et al Mst4 and Ezrin Induce Brush Borders Downstream of the Lkb1/Strad/Mo25 Polarization Complex Developmental Cell 16 (2009) 551-562[Application: WB ICC]2. Romancer ML Treilleux I Leconte N et al Regulation of Estrogen Rapid Signaling through Arginine Methylation by PRMT1 Molecular Cell 31 (2008) 212-221[Application: WB]3. Han ZF Guo L Wang HY et al Structural Basis for the Specific Recognition of Methylated Histone H3 Lysine 4 by the WD-40 Protein WDR5 Molecular Cell 22 (2006) 137-144[Application: ]4. Nevlani P Santhanam S Trotta R et al The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABLregulated SET protein Cancer Cell 8 (2005) 355-368[Application: IP]5. Alexandru G Graumann J Smith GT et al UBXD7 Binds Multiple Ubiquitin Ligases and Implicates p97 in HIF1a Turnover Cell 134 (2008) 804-816[Application: IP]6. Cao GD Xing J Xiao X et al Critical Role of Calpain I in Mitochondrial Release of Apoptosis-Inducing Factor in Ischemic Neuronal Injury The Journal of Neuroscience 27 (2007) 9278 -9293[Application: WB]7. Ishimura R Martin GR and Ackerman SL Loss of Apoptosis-Inducing Factor Results in Cell-Type-Specific Neurogenesis Defects The Journal of Neuroscience 28 (2008) 4938-4948[Application: IHC]8. Bajt ML Farhood A Lemasters JJ et al Mitochondrial Bax Translocation Accelerates DNA Fragmentation and Cell Necrosis in a Murine Model of Acetaminophen Hepatotoxicity JPET 324 (2008) 8-14[Application: WB]9. Yu SW Andrabi SA Wang HM et al Apoptosis-inducing factor mediates poly(ADP-ribose) (PAR) polymer-induced cell death PNAS 103 (2006) 18314 -18319[Application: ICC]10. Lin CY Wu HY Wang PL et al AIF Int J Biochem Cell Biol 42 (2010) 98-105[Application: WB]11. Shen H Hu X Liu C et al Ethyl pyruvate protects against hypoxic-ischemic brain injury via anti-cell death and anti-inflammatory mechanisms Neurobiol Dis 37 (2010) 711-722[Application: WB]12. Chen X Gong J Zeng H et al MicroRNA145 Targets BNIP3 and Suppresses Prostate Cancer Progression Cancer Res 70 (2010) 2728-27381. Susin S. A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397(6718): 4412. Daugas E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476(3): 1183. Daugas E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14(5):7294. Susin S. A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192(4): 5715. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487