Caspase-7 (pro)

GWB-444FFF

A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7 was used as immunogen. The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.

WB: 1:1 000IHC: 1:50ICC: 1:50. Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1 Mch3 ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1 2 3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation Caspase-7 is translocated to the mitochondria and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2 3) and endoplasmic reticular-specific substrate sterol regulatory element-binding protein 1 (4). 1. Duan H. et al. ICE-LAP3 a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J. Biol. Chem. 271:2. Lippke J. A. et al. Identification and characterization of CPP32/Mch2 homolog 1 a novel cysteine protease similar to CPP32. J. Biol. Chem. 271: 18253. Fernandes-Alnemri T. et al. Mch3 a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res. 55: 60454. Chandler J. M. et al. Different Subcellular Distribution of Caspase-3 and Caspase-7 following Fas-induced Apoptosis in Mouse Liver. J. Biol. Chem. 273: 108155. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487