Phsopho-HSP27 (S82)

GWB-8204A3

A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human Hsp27 was used as immunogen. The antibody only detects Hsp27 phosphorylated on Serine 82.

WB: 1:2000IHC: 1:250ICC: 1:100IP: 1:30. Heat shock protein (HSP) 27 is one of the small HSPs and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock or estrogen stimulation in MCF-7 cells (1 2). HSP27 is phosphorylated at serines 15 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3 4). 1. Momose S Tamaru JI Kishi H et al Hyperactivated STAT3 in ALK-positive diffuse large B-cell lymphoma with clathrin-ALK fusion Human Pathology 40 (2009) 75-82[Application: ICC]2. Momose S Tamaru JI Kishi H et al Hyperactivated STAT3 in ALK-positive diffuse large B-cell lymphoma with clathrin-ALK fusion Human Pathology 40 (2009) 75-82[Application: IHC]3. Diestel A Roessler J Berger F et al Hypothermia downregulates inflammation but enhances IL-6 secretion by stimulated endothelial cells Cryobiology 57 (2008) 216-222[Application: WB]4. Hong Z Zhang QY Liu J et al Phosphoproteome Study Reveals Hsp27 as a Novel Signaling Molecule Involved in GDNF-Induced Neurite Outgrowth J. Proteome Res. 8 (2009) 2768-2787[Application: WB]5. Chilosi M Zamà ² A Doglioni C et al Migratory marker expression in fibroblast foci of idiopathic pulmonary fibrosis Respir Res 7 (2006) :95[Application: IHC]1. Mendelsohn M. E. et al. The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Proc Natl Acad Sci U S A. 88: 112122. Faucher C. et al. The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein. J. Biol Chem. 268:151683. Landry J. et al. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II. J. Biol. Chem. 267: 7944. Rouse J. et al. A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins. Cell 78: 1027-1037 (1994). 5. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487