Phospho-Jak2 (pY1007/1008)

GWB-7062C6

A synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.

WB: 1:5 000-10 000IHC: 1:50-100ICC: 1:50-100IP: 1:50. Jak2 is a member of the Janus family tyrosine kinases (Jaks) whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins one class of which is the signal transducers and activators of transcription (STATs). Activated STATs then translocate to the nucleus to mediate gene transcription (1 2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2 and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4). 1. Leonard W. J. and J. J. O2. O\' Shea J. J. et al. Advances in the understanding of cytokine signal transduction: the role of Jaks and STATs in immunoregulation and the pathogenesis of immunodeficiency. J Clin Immunol. 17: 4313. Gauzzi M. C. et al. Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase. J. Biol. Chem. 271: 204944. Peeters P. et al. Fusion of TEL the ETS-variant gene 6 (ETV6) to the receptor-associated kinase JAK2 as a result of t(9; 12) in a lymphoid and t(9; 15; 12) in a myeloid leukemia. Blood 90: 25355. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487