ATF-2 (C-term)

GWB-B45520

A synthetic peptide corresponding to residues in C-terminus of human ATF2 was used as immunogen. The antibody does not cross-react with other ATF family members.

WB: 1:1 000 - 10 000IHC: 1:250-500ICC: 1:250-500IP: 1:60. ATF-2 (activating transcription factor-2 CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress including genotoxic agents inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate in response to cellular stress ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). 1. Maekawa T. et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8: 20232. Gupta S. et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 3893. van Dam H. et al. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J. 14: 17984. Livingstone C. et al. ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14: 17855. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487