p73

GWB-264FBB

A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 kDa but is detected at 63kDa.

WB: 1:500-2 000IHC: 1:100-250ICC: 1:150IP: 1:50. p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over- expression (2) Unlike p53 p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63 p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (a beta) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5). 1. Waldele K Silbermann K Schneider G et al Requirement of the human T-cell leukemia virus (HTLV-1) tax-stimulated HIAP-1 gene for the survival of transformed lymphocytes Blood 107 (2006) 4491-4499[Application: WB]2. Mashukova A Oriolo AS Wald FA et al Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones J. Cell Sci. 122 (2009) 2491 - 2503[Application: WB]3. Sasaki Y Negishi H Koyama R et al P53 family members regulate the expression of the apolipoprotein D gene J. Biol. Chem. 284 (2009) 872 - 883[Application: WB]1. Kaghad et al. Cell 90: 809-819 1997. 2. Yang A. Kaghad M. Wang Y. Gillett E. Fleming M. D. Dotsch V. Andrews N. C. Caput D. and McKeon F. (1998) Mol. Cell 2 305-3163. Marin MC Jost CA DeCaprio JA Caput D Kaelin WG Jr. Mol Cell Biol 1998; 18:6316-244. Levrero M. De Laurenzi V. Costanzo A. Gong J. Melino G. and Wang J. Y. (1999) Cell Death Differ. 6 1146-11535. Marin et al. Nature Genet. 25: 47-54 2000. 6. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487