NAK / TBK1 (N-term)

GWB-ADC799

A synthetic peptide corresponding to residues in the N-term of human NAK was used as immunogen.

WB: 1:1 000 - 10 000IHC: 1:50ICC: 1:250-500FC: 1:40. NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor-associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2) NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK TRAF-2 and TRIF (4). 1. Jin CY Yang YA Anver MR et al Smad Ubiquitination Regulatory Factor 2 Promotes Metastasis of Breast Cancer Cells by Enhancing Migration and Invasiveness Cancer Res 69 (2009) 735-740[Application: WB]2. Panomketa P Splitterc G Harmsc J et al TBK1 does not play a role in the control of in vitro Burkholderia pseudomallei growth Transactions of the Royal Society of Tropical Medicine and Hygiene 102/S1 (2008) S95 - S100[Application: WB]1. Pomerantz J. L. ; Baltimore D. : EMBO J. 18: 6694-6704 1999. 2. Karin M. (1999) J. Biol. Chem. 274 273393. Tojima Y. Fujimoto A. Delhase M. Chen Y. Hatakeyama S. Nakayama K. Kaneko Y. Nimura Y. Motoyama N. Ikeda K. Karin M. and Nakanishi M. (2000) Nature 404 7784. Nomura F. Kawai T. Nakanishi K. and Akira S. (2000) Genes Cells 5 191-2025. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487