Phospho-EGFR (pY1068)

GWB-333752

A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as an immunogen.

WB: 1:500 - 1000IHC: 1:250 - 500ICC: 1:250 - 500. Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that when activated by epidermal growth factor (EGF) affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR Tyr 1068 Tyr 1148 and Tyr 1173 are the major sites of autophosphorylation which occurs as a result of EGF binding (3). EGFR overexpression is exhibited various cancer such as glioma colorectal carcinoma breast carcinoma and head and neck carcinoma (4). The phosphorylation level of EGFR is considered one of the important predicators for outcome in non-small cell lung cancer therapy (5). 1. Hunter T. et al. Nature 311: 4142. Reynolds FH et al. Nature 292: 2593. Helin K et al. Oncogene 6: 8254. Christensen ME et al. Danish Med Bull 45(2):121-134 1998. 5. Kanematsu T et al. Oncol Res 13(5):289-298 20036. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487