Stathmin

GWB-5361D1

A synthetic peptide corresponding to residues in C-terminus of human Stathmin was used as immunogen.

WB: 1:50 000 - 200 000IHC: 1:250-1:500ICC: 1:100-1:250FC: 1:100IP: 1:50. Stathmin (oncoprotein 18 op18) is a ubiquitous cytosolic phosphoprotein with various regulatory functions in cell proliferation differentiation signaling and activation (1). In particular stathmin is involved in the regulation of tubulin dynamics through inhibition of microtubule formation and/or microtubule depolymerization. Stathmin interacts with soulable tubulins ( tubulin) resulting in the formation of T2S complex which sequesters free tubulin impeding tubulin formation (2). Stathmin activity is regulated through phosphorylation at Ser16 Ser25 Ser38 and Ser63 by various protein kinases (e. g. MAP-kinase p34cdc2 kinase) weakening stathmin\' s affinity to tubulin (3). A mutation on stathmin can lead to excess build up of mitotic spindle and with possible consequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also known as the generic member of a protein family which includes neural proteins SCG10 SCLIP and RB3/RB3\' /RB3\" . All members in this family exhibits tubulin binding ability. 1. Nery FC Zeng J Niland BP et al TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton J Cell Sci 121 (2008) 3476-3486[Application: WB]2. Kim WY Chang DJ Hennessy B et al A Novel Derivative of the Natural Agent Deguelin for Cancer Chemoprevention and Therapy Cancer Prev Res 1 (2008) 577-587[Application: WB]1. Cassimeris L et al. Curr Opin in Cell Bio. 14:18-24 2002. 2. Clement MJ et al. Biochemistry. 44:14616-14625 2005. 3. Jourdain L et al. Biochemistry 36:10817-10821 1997. 4. Moreno FJ Avila J Mol Cell Biochem 183: 201-209 19985. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487