Cytokeratin 8

GWB-EB41C9

A synthetic pathway corresponding to residues on the C-terminus of human CK8 was used as an immunogen.

WB: 1:25 000 - 50 000IHC: 1:250 - 500ICC: 1:100 - 250FC: 1:20IP: 1:70. Keratins the intermediate filament proteins of epithelial cells connect to desmosomes the cell-cell adhesion structures at the surface membrane. Among the various intermediate filament (IF) proteins cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis is the only type-II CK occurring in many simple epithelial cells and can also be synthesized in certain non-epithelial cells (1). Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia as found in the liver pancreas and intestine (2). While K8/K18 are essential for maintaining structural integrity there is accumulating evidence indicating that they also exert non-mechanical functions. It has been reported that K8/K18-free hepatocytes from K8-null mice are more sensitive to Fas-mediated apoptosis in line with an increased Fas density at the cell surface and an altered c-Flip regulation of the anti-apoptotic ERK1/2 signaling pathway (3). 1. Krauss S et al. Gene 86(2):241-9 19902. Ku NO et al. J. Clin Invest 99(1):19-23 1997. 3. Galarneau L et al. Exp Cell Res. 313(1):179-94 20074. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487