ATM Phospho (pS1981)

GWB-10B9D7

A synthetic peptide corresponding to residues surrounding serine 1981 of human ATM was used as an immunogen.

WB: 1:2000 - 10000IHC: 1:100 - 250ICC: 1:100 - 250IP: 1:40. ATM is a nuclear phosphoprotein involved in Ataxia-telangiectasia (A-T) an autosomal recessive disorder characterized by cerebellar ataxia immune deficiencies increased cancer predisposition chromosomal instability and radiation sensitivity (1-2). This large protein (370 kDa) is involved in genome stability cellular responses to DNA damage and cell cycle control. Autophosphorylation at serine 1981 on ATM is required during ATM kinase activation initiated by double strand breaks in genome. After serine 1981 autophosphorylation ATM phosphorylates downstream proteins (e. g. p53 Chk2) leading to cell cycle arrest and DNA repair (3). More than 100 mutations have been identified so far and are expected to inactivate the ATM protein by truncation or large deletions (4). Defects in ATM contribute to various B cell and T cell Leukemia (5). 1. Dauber B Martà ­ nez-Sobrido L Schneider J et al Influenza B Virus Ribonucleoprotein Is a Potent Activator of the Antiviral Kinase PKR PLoS Pathog. 5 (2009) e1000473[Application: IP]2. Hansen A Finger TE Is TrpM5 a reliable marker for chemosensory cells? Multiple types of microvillous cells in the main olfactory epithelium of mice BMC Neuroscience 2008 9:115[Application: ]3. Togano T Sasaki M Watanabe M et al Induction of oncogene addiction shift to NF-Î º B by camptothecin in solid tumor cells Biochem Biophys Res Commun 390 (2009) 60-64[Application: WB]4. Goff JP Shields DS Seki M et al ATM (pS1981) Radiat Res 172 (2009) 165-174[Application: IP]5. Beyrouthy MJ Garner KM Hever MP et al ATM (pS1981) Cancer Res. 69 (2009) 9360-93666. Noon AT Shibata A Rief N et al 53BP1-dependent robust localized KAPâ ??1 phosphorylation is essential for heterochromatic DNA double-strand break repair Nat Cell Biol 12 (2010) 177-184[Application: ICC]7. Pires IM Bencokova Z Milani M et al Effects of Acute versus Chronic Hypoxia on DNA Damage Responses and Genomic Instability Cancer Res 70 (2010) 925-9358. Gagou ME Zuazua-Villar P Meuth M Enhanced H2AX Phosphorylation DNA Replication Fork Arrest and Cell Death in the Absence of Chk1 Mol Biol Cell 21 (2010) 739-7529. Williamson CT Muzik H Turhan AG et al ATM Deficiency Sensitizes Mantle Cell Lymphoma Cells to Poly(ADP-Ribose) Polymerase-1 Inhibitors Mol Cancer Ther 9 (2010) 347-35710. Kobayashi J Kato A Ota Y et al Bisbenzamidine derivative pentamidine represses DNA damage response through inhibition of histone H2A acetylation Mol Cancer 9 (201) :34[Application: ICC]1. Harnden D. G. ; Int. J. Radiat. Biol. 66:S13-S19 1994. 2. Lavin MF Shiloh Y. Ann. Rev. Imm. 15:177-202 19973. Goldstine JV et al. DNA Repair 5:432-443 2006. 4. Savitsky K et al. Hum. Mol. Gen. 4:2025-2032 1995. 5. Stankovic T. et al. Lancet 353:26-29. 1999. 6. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487