Transportin 1 [D45]

GWB-CC1DEE

IgG1

Human

Cytoplasm nucleoplasm and nuclear rim.

Protein A purified from tissue culture supernatant. Storage

ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring Ran-dependent mechanism. At the nucleoplasmic side of the NPC Ran binds to the importin the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP) A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro mediates nuclear import of H2A H2B H3 and H4 histones and SRP19. In case of HIV-1 infection binds and mediates the nuclear import of HIV-1 Rev.

Cytoplasm. Nucleus.

Contains 8 HEAT repeats.