MYO6 [MUD-19]

MUD-19

Synthetic peptide corresponding to amino acids 291-302 of Human MYO6.

A431 whole cell lysate

Cell Membrane Cytoplasmic and Golgi Apparatus

15% Sodium Azide; Constituents: 1% BSA 0. 01M PBS. pH 7. 4

Cell Membrane Cytoplasmic and Golgi Apparatus Myosins belong to a superfamily of actin based motor proteins comprising at least 15 or more classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain a light chain binding neck region a coil-coiled region and a highly conserved C-terminal domain. At the converter region between the catalytic head and the neck region of myosin VI there is a characteristic linker of approximately 50 amino acids. Native myosin VI is apparently a two-headed dimer of the heavy chains with each heavy chain bound to calmodulin light chain. Myosin VI is a unique reverse actin motor in vitro i. e. to display motility towards the pointed (minus) ends of actin filaments a direction opposite to all other currently known myosins.

Homodimer. Binding to calmodulin through a unique insert not found in other myosins located in the neck region between the motor domain and the IQ domain appears to contribute to the directionality reversal. This interaction occurs only if the C-terminal lobe of calmodulin is occupied by calcium. Interaction with F-actin/ACTN1 occurs only at the apical brush border domain of the proximal tubule cells (By similarity). Interacts with DAB2. In vitro the C-terminal globular tail binds a C-terminal region of DAB2. Interacts with CFTR. Forms a complex with CFTR and DAB2 in the apical membrane of epithelial cells.

Isoform 3: Cytoplasmic vesicle clathrin-coated vesicle membrane.

Expressed in most tissues examined including heart brain placenta pancreas spleen thymus prostate testis ovary small intestine and colon. Highest levels in brain pancreas testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2 containing the small insert and isoform 4 containing neither insert are expressed in unpolarized epithelial cells.

Phosphorylation in the motor domain induced by EGF results in translocation of MYO6 from the cell surface to membrane ruffles and affects F-actin dynamics. Phosphorylated in vitro by p21-activated kinase (PAK) (By similarity).

Defects in MYO6 are the cause of non-syndromic sensorineural deafness autosomal recessive type 37 (DFNB37) [MIM:607821].

Contains 1 IQ domain.