S100 beta [SH-B4]

GWB-4F78D4

IgG1

Bovine

Human tongue tissue slide (IHC-P)

Ascites Storage

Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependent dilution. Predicted

Cytoplasmic S100 is a set of small thermolabile highly acidic dimer proteins of approx. 20 kDa widely distributed in different tissues. Dimeric combinations of two chains; the alpha chain (93 a. a. 10. 4 kDa) and the beta chain (91 a. a. 10. 5 kDa) form the three known subtypes of S100 [S100ao (alpha alpha) S100a (alpha beta) and S100b (beta beta). Although there is slight variation in the primary structure in different species the S100 molecule is markedly conserved in the amino acid sequence and the protein extracted from different organs of the same species is identical. The alpha and beta chains are 58% homologous (54 a. a. ) and both have divalent cation binding sites situated toward the carboxy terminus and apparently similar functional features. S100 can be grouped with other calcium binding proteins to which it has a significant sequence homology particularly around the calcium binding domain such as calmodulin parvalbumin intestinal calcium binding protein myosin light chain and troponin-C. Hence S100 is a calcium modulated protein that binds calcium and zinc ions reversibly at physiologic pH and ionic strength followed by a conformational change in the molecule. S100 is considered to be a cell-growth regulator but other functions have been suggested e. g. increasing the membrane permeability to cations under physiologic conditions stimulation of nucleolar RNA polymerase activity interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes. Human S100 containing cells are subdivided to three groups S100b containing cells (such as Schwann cells pituicytes of the neurohypophysis Langerhaní s cells and interdigitating cells) S100a containing cells (such as glial cells and melanocytes) and S100ao containing cells (such as neurons ganglion cells slow skeletal muscle cells cardiac cells monocytes and some macrophages). Although the tissue distribution of S100 is too broad to conform to a single histogenetic pattern it is sufficiently restricted that the localization of this protein is useful in the differential diagnosis of neoplasms and proliferative processes. Monoclonal antibody reacting specifically with the beta subunit of S100 may be use to distinguish malignant melanoma from undifferentiated carcinoma or lymphoma and to distinguish leiomyomas from schwannomas and their counterparts in the gastrointestinal tract.

Dimer of either two alpha chains or two beta chains or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1.

Although predominant among the water-soluble brain proteins S100 is also found in a variety of other tissues.

Belongs to the S-101 family.