SV40 T-antigen [PAb416]

PAb416

Full length protein (purified) (SV40).

SV80 or SV-T2 cells

Nuclear

50mM Sodium phosphate 0. 2% gelatin with 0. 1% sodium azide as preservative

82 kDa and 20 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Cellular

Isoform large T antigen plays a role in virion assembly. Autoregulates the synthesis of the viral early mRNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1 thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1 leading to activation of transcription.

Isoform large T antigen forms homohexamers in the presence of ATP. Interacts with host HDAC1. Interacts (via LXCXE domain) with host RB1; the interaction induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1\' s activity. Interacts (via LXCXE domain) with host pRB-related proteins RBL1 and RBL2. Interacts (via C-terminus) with host TOP1 and POLA1 allowing DNA replication. Interacts with host TP53 inhibiting TP53 binding to DNA. Interacts with host preinitiation complex components TBP TFIIA and TFIID to regulate transcription initiation.

The J domain is essential for multiple viral activities including virion assembly viral DNA replication transformation and transcriptional activation.

The origin-binding domain (T-ag OBD) interacts specifically with several pentameric sequences 5\' -GAGGC-3\' in the SV40 origin of DNA replication.

The ATP binding/ATPase domain is required for proper hexamer assembly and helicase activity.

O-Glycosylated near the C-terminal region.

Contains 1 J domain.

Contains 1 T-ag D1-type zinc finger.