Cytokeratin 5 + 14 [LH8]

LH8

The 50 000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.

Detected in the basal layer lowered within the more apically located layers specifically in the stratum spinosum stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

Basal layer

None. Constituents: PBS

Basal layer Myeloma: Sp2/0-Ag14 Cytokeratins (CK) are intermediate filaments of epithelial cells both in keratinizing tissue (ie. skin) and non-keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8. 5 can be identified using two dimensional gel electrophoresis. Biochemically most members of the CK family fall into one of two classes type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5 keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering milia formation dystrophic nails and mucous membrane involvement.

Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.

Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and \' mottled\' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).