AIB1 [AC3]

ArtNr	20-272-193215
Hersteller	GENWAY
Menge	0.1 mg
Kategorie	Oncology Antibodies By Cell Type Carcinoma Western Blot Primary Antibodies By Organ Breast Cancer
Typ	Antibody
Applikationen	WB
Clon	AC3
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-915022
Similar products	20-272-193215
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Genway ID:	GWB-915022
Clone:	AC3
Isotype:	IgG1Light chain: kappa
Immunogen:	A synthetic peptide to the C-terminal end of human AIB1 conjugated to KLH.
Antigen Species:	Human
Specificity:	AIB1-AC3 recognizes AIB1 a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 155kDa.
Positive Control:	MCF-7 nuclear extracts
Target:	AIB1
Concentration:	1 mg/ml Storage
Buffer:	Phosphate-buffered saline pH7. 4 containing no preservatives
Application Note:	For WB: Use at a concentration of 1-5 µ g/ml. Predicated
Molecular Weight:	155 kDa. Not tested in other applications. Can be blocked with AIB1 (). Optimal dilutions/concentrations should be determined by the researcher. Myeloma: P3x63-Ag8. 653 AIB1-AC3 recognizes AIB1 a member of the SRC-1 family of nuclear receptor coactivators with an apparent molecular weight of 160kDa. The AIB1 gene has been mapped to region 20q12 and has been shown to be amplified in breast tumors and pancreatic cancer cell lines. AIB1 interacts in a ligand-dependent fashion with the estrogen receptor to enhance estrogen-dependent transcriptional gene activation.
Function:	Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors such as for steroids (GR and ER) retinoids (RARs and RXRs) thyroid hormone (TRs) vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity).
Catalytic Activity:	Acetyl-CoA + histone = CoA + acetylhistone.
Enzyme Regulation:	Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones and the TNF cytokine respectively. TNF stimulation probably enhances phosphorylation which in turn activates coactivator function. In contrast acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors (By similarity).
Subunit:	Interacts with the histone acetyltransferase protein CREBBP. These two proteins are present in a complex containing NCOA2 IKKA IKKB and IKBKG. Interacts with PCAF and NR3C1 (By similarity). Interacts with CARM1. Interacts with CASP8AP2. Interacts with ATAD2 and this interaction is enhanced by estradiol (By similarity).
Subcellular Location:	Cytoplasm (By similarity). Nucleus (By similarity). Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation it translocates from the cytoplasm to the nucleus (By similarity).
Tissue Specificity:	Not expressed in all steroid sensitive tissues. Highly expressed in the female reproductive system in both oocyte and smooth muscle cells of the oviduct but not expressed in the uterine endometrium. Highly expressed in mammary glands. Expressed moderately in smooth muscle cells of both blood vessels and intestines and weakly expressed in hepatocytes. In brain highly expressed in neurons of the hyppocampus and in mitral cell and granule layers of the olfactory bulb. Expressed moderately in the internal layer of cerebellum. Not expressed in the spinal chord cardiac muscle skeletal muscle thymus and pancreas.
Domain:	Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors and constitute the RID domain (Receptor-interacting domain) (By similarity).
Ptm:	Acetylated by CREBBP. Acetylation occurs in the RID domain and disrupts the interaction with nuclear receptors and regulates its function (By similarity).
Ptm:	Methylated by CARM1.
Ptm:	Phosphorylated by IKK complex. Regulated its function (By similarity).
Miscellaneous:	Defects in NCOA3 result in diverse phenotype of postnatal growth retardation such as dwarfism delayed puberty abnormal reproductive fucntion and mammary gland growth retardation.
Similarity:	Belongs to the SRC/p160 nuclear receptor coactivator family.
Similarity:	Contains 1 basic helix-loop-helix (bHLH) domain.
Similarity:	Contains 1 PAS (PER-ARNT-SIM) domain.

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