FEN1 [4E7]

GWB-8C5945

IgG1Light chain: kappa

Human

FEN1

1 mg/ml

Phosphate-buffered saline pH 7. 4 containing no preservatives

43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Target protein

Nuclear Expression system: pET Myeloma: NS1 FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5\' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Interacts with PCNA. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300.

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.