F-actin [NH3]

NH3

Human monocytes and U937 cell line

This recognises human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly and gives granular localised nuclear staining of all cell types.

Lot specific and is stated on the vial

Phosphate buffered saline pH 7. 4 containing 0. 09% sodium azide

Use at a dilution of 1:5 - 1:25. For IHC-Fr: Use at an assay dependent dilution. Not tested in other applications. Optimal concentrations/dilutions should be determined by the researcher. Myeloma: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Filamentous (F) actin is a principle cytoskeletal element in some non-muscle cells including polymorphonuclear leukocytes (PMNs).

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Component of the BAF complex which includes at least actin (ACTB) ARID1A ARID1B/BAF250 SMARCA2 SMARCA4/BRG1 ACTL6A/BAF53 ACTL6B/BAF53B SMARCE1/BAF57 SMARCC1/BAF155 SMARCC2/BAF170 SMARCB1/SNF5/INI1 and one or more of SMARCD1/BAF60A SMARCD2/BAF60B or SMARCD3/BAF60C. In muscle cells the BAF complex also contains DPF3. Found in a complex with XPO6 Ran ACTB and PFN1. Component of a complex composed at least of ACTB AP2M1 AP2A1 AP2A2 MEGF10 and VIM. Interacts with XPO6.

Defects in ACTB are a cause of juvenile-onset dystonia [MIM:607371]. Dystonia is a movement disorder with a neurological basis due to disordered tonicity of muscle. It is characterized by sustained involuntary muscle contractions that cause abnormal postures and repetitive movements. It may affect muscles throughout the body (generalized) in certain parts of the body (segmental) or may be confined to particular muscles or muscle groups (focal). Individuals with juvenile-onset dystonia due to ACTB mutations present a complicated phenotype that includes progressive generalized dopa-unresponsive dystonia developmental malformations and sensory hearing loss.

Belongs to the actin family.