Cytokeratin 19 [JZ202]

GWB-401C49

IgG1Light chain: kappa

Human

Cytokeratin 19

tissue culture supernatant Storage

For ICC: Use at an assay dependent dilution. For IHC-Fr: Use at a dilution of 1:50-1:100. For IHC-P: Use at a dilution of 1:50-1:100 using a streptavidin-Biotin system or polymer system. Antigen retrieval by boiling the tissue in 10 mM citrate pH 6. 0 for 10-15 minutes followed by cooling for 10-15 minutes is recommended. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Involved in the organization of myofibers. Together with KRT8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells bile ducts gastrointestinal tract bladder urothelium oral epithelia esophagus ectocervical epithelium (at protein level). Expressed in epidermal basal cells in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. Developmental Stage: Present in hair follicles at all stages of development.

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).