NDC80 homolog kinetochore complex component (S.cerevisiae) (NDC80)

GWB-447FDE

Acts as a component of the essential kinetochore-associated NDC80 complex which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.

Nucleus. Kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. Developmental Stage: Expression peaks in mitosis.

Belongs to the NDC80/HEC1 family. Summary: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved. [supplied by OMIM]. [1] Wei R. R. Al-Bassam J. and Harrison S. C. et al. The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment[2] DeLuca J. G. Gall W. E. Ciferri C. Cimini D. Musacchio A. and Salmon E. D. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1[3] Lin Y. T. Chen Y. Wu G. and Lee W. H. et al. Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control[4] Nousiainen M. Sillje H. H. Sauer G. Nigg E. A. and Korner R. et al. Phosphoproteome analysis of the human mitotic spindle[5] Ahonen L. J. Kallio M. J. Daum J. R. Bolton M. Manke I. A. Yaffe M. B. Stukenberg P. T. and Gorbsky G. J. Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores[6] Chen Y. Riley D. J. Chen P. -L. Lee W. -H. HEC a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. [7] Chen Y. Sharp Z. D. Lee W. -H. HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins. [8] Zheng L. Chen Y. Lee W. -H. Hec1p an evolutionarily conserved coiled-coil protein modulates chromosome segregation through interaction with SMC proteins. [9] Zheng L. Chen Y. Riley D. J. Chen P. -L. Lee W. -H. Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p. [10] Chen Y. Riley D. J. Zheng L. Chen P. -L. Lee W. -H. Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.