Influenza B (Nucleoprotein)

IgG2b

Monoclonal Antibodies to Infectious Agents and Toxins

PBS pH 7. 5Applications Notes : Suitable for use in ELISA Western blotting and Immunofluorescence. Prepare working dilution only prior to immediate use. Each laboratory should determine an optimum working titer for use in its particular application. Suitability for use in Flow cytometry Immunohistology Immunoprecipitation or Radioimmunoassay has not been determined but use in such assays should not necessarily be excluded.

Encapsidates the negative strand viral RNA protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein\' s nuclear localization signals. Soon after a virion infects a new cell M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein\' s nuclear localization signals targeting the RNP to the nucleus.

Virion (Potential). Nucleus.

Belongs to the influenza viruses nucleoprotein family.