MOUSE ANTI V5-TAG

GWB-379A6A

IgG1

Phosphate buffered saline pH7. 4

A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.

Has been used to detect recombinant proteins some of which include transmembrane and secreted proteins which have been tagged with this epitope. Usually a 14 amino acid tag has been added to the recombinant proteins although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)