CD33

CD33NCBI

CD33

Human AML cells

This product recognises the human CD33 cell surface glycoprotein a 67kD molecule expressed by monocytes granulocyte precursors myeloid progenitor cells and myeloid leukaemias.

Phosphate buffered saline pH7. 4

Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Suggested

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha2 6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Cell membrane; Single-pass type I membrane protein.

Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Favaloro. E. J. et al. (1987) Characterisation of monoclonal antibodies to the human myeloid-specific antigen \' gp67\' (CD-33). Dis Markers. 5: 215-225. 2. Favaloro. E. J. et al. (1988) Further characterisation of myeloid antigens (\' gp160. 95\' . \' gp150\' and \' gp67\' ): Investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b. CD-13 and CD33. Br. J. Haematol. 69: 163-171.