V5-TAG

V5-TAG

SV5-Pk1

Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Suggested

Belongs to the G-protein coupled receptor 1 family [view classification]. 1. Southern. J. A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. 2. Hanke. T. et al. (1992) Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen. 3. Randall. R. E. et al. (1993) Two-tag purification of recombinant proteins for the construction of solid matrix-antibody antigen (SMAA) complexes as vaccines. 4. Randall. R. E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins; comparison of antigen and antibody-antigen complexes for immune priming. 5. Hanke. T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding 6. Jaffray. E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65 7. Rodriguez. M. S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein. 8. Arenzana-Seisdedos. F. et al. (1995) Inducible nuclear expression of newly synthesised IkBa negatively regulates DNA binding and transcriptional activities of NF-kB. Mol. Cell. Biol. 15: 2689-2696. 9. Hirst. K. et al. (1994) The transcription factor. the Cdk. its cyclin and their regulator: directing the transcriptional response to a nutritional signal 10. Dunn. C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. 11. Young. D. F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells 12. Stuart A. MacNeill (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase {delta} is responsible for direct interaction with the B-subunit