INFLUENZA B NUCLEOPROTEIN

GWB-C7A95A

INFLUENZA B NUCLEOPROTEIN

Influenza B/Lee/40 and B/Singapore/-222/79 viruses.

This product specifically recognises an epitope within the nucleoprotein of influenza B virus. The influenza viruses classified as type A B and C are members of the Orthomyxoviridae family which differ in their epidemiology and host ranges and lack serological cross-reactivity of their internal components especially their matrix proteins and nucleoprotein. Influenza B virus is a slow-mutating single stranded RNA virus subject to antigenic drift which although enough to prevent lasting immunity prevents influenza B from causing pandemics. This product can be used in influenza B IFA typing in conjunction with (clone B114).

Phosphate buffered saline pH7. 5

Encapsidates the negative strand viral RNA protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein\' s nuclear localization signals. Soon after a virion infects a new cell M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein\' s nuclear localization signals targeting the RNP to the nucleus (By similarity).

Virion (Potential). Nucleus.

Belongs to the influenza viruses nucleoprotein family. 1. Walls. H. H. et al. (1986) Characterization and evalution of monoclonal antibodies developed for typing Influenza A and Influenza B viruses. 2. Zhirnov. O. P. et al. (1999) Caspase-dependent N-terminal cleavage of Influenza virus nucleocapsid protein in infected cells