CD36

GWB-4FC684

CD36

Human tonsil and peripheral blood mononuclear cells

This product recognises the CD36 antigen an 88kD cell surface glycoprotein expressed by platelets monocytes/macrophages and also by some microvascular endothelium and erythroid precursors. CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. This product partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team. This clone is also reported to block interaction of oxidised low-density lipoprotein (OXLDL) with CD36 (6). We recommend the use of for this purpose.

Phosphate buffered saline

Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested

Seems to have numerous potential physiological functions. Binds to collagen thrombospondin anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport.

N-glycosylated and O-glycosylated with a ratio of 2:1.

Defects in CD36 are the cause of platelet glycoprotein IV deficiency [MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets but expression in monocytes/macrophages is near normal.

Belongs to the CD36 family. 1. Hogg. N. (1985) Mononuclear phagocytes of normal and rheumatoid synovial membrane identified by monoclonal antibodies. 2. Greenwalt. D. E. et al. (1992) Membrane glycoprotein CD36: a review of its roles in adherence. signal transduction and transfusion medicine. 3. Endemann. G. et al. (1993) CD36 is a receptor for oxidized low density lipoprotein. 4. Sugihara. K. et al. (1992) Thrombospondin mediates adherence of CD36+ sickle reticulocytes to endothelial cells. 5. Hogg. N. et al. (1984) Monoclonal antibodies specific for human monocytes. granulocytes and endothelium. 6. Maxeiner. H. et al. (1998) Complementary roles for scavenger receptor A and CD36 of human monocyte-derived macrophages in adhesion to surfaces coated with oxidized-low density lipoproteins and secretion of H2O2.