Tubulin b

GWB-81CCD9

MAD109 cell. Skin or lung

A synthetic peptide derived from aa 416-430 of human tubulin beta. Microtubules the major cytoskeletal elements found in all eukaryotic cells consist of Tubulin which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis intracellular transport ciliary and flagellar bending and structural support of the cytoskeleton. This antibody does not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Ab-3 does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.