Mouse Anti-Rabbit RLA-DQ

Mouse (BALB/c) IgG2a kappa

Rabbit spleen cells

Rabbit RLA-DQ chain (Mr ~35 kDa)MAb 2C4 reacts with RLA-DQ-transfected cells but not with RLA-DR-transfected cells^2. In normal adult rabbits the antibody reacts with 30-60% of peripheral blood leukocytes. ^2 3 It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i. e. infiltrating macrophages) in inflamed synovium. ^4 DQ transcripts have been detected in bone marrow and spleen and to a lesser extent in appendix lymph nodes and other organs. ^5

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes or clothing. Wear eye or face protection when handling. If skin or eye contact occurs wash with copious amounts of water. If ingested or inhaled contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. Lobel S. A. and K. L. Knight. 1984. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51:35-43. Spieker-Polet H. N. Sittisombut P. -C. Yam and K. L. Knight. 1990. Rabbit major histocompatibility complex IV. Expression of major histocompatibility complex class II genes. J. Immunogenetics 17:123-132. nology Associates Inc. Unpublished observations. Wilkinson J. M. G. McDonald S. Smith J. Galea-Lauri J. Lewthwaite B. Henderson and P. A. Revell. 1993. Immunohistochemical identification of leucocyte populations in normal tissue and inflamed synovium of the rabbit. J. Pathol. 170:315-320. Kulaga H. J. -A. Sogn J. D. Weissman P. N. Marche C. LeGuern E. O. Long and T. J. Kindt. 1987. Expression patterns of MHC class II genes in rabbit tissues indicate close homology to human counterparts. J. Immunol. 139:587-592.