Helicobacter Pylori IgG Enzyme Immunoassay Test Kit

IgG Antibodies to Helicobacter pyloriSample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Helicobacter pylori IgG Test Kit is intended for use in evaluating the serologic status to H. pylori infection in specimens with gastrointestinal symptoms. Introduction: Helicobacter pylori is a spiral bacterium cultured from human gastric mucosa discovered by B. J. Marshall in 1982. Studies have indicated that the presence of H. pylori is associated with a variety of gastrointestinal diseases including gastritis duodenal and gastric ulcers non-ulcer dyspepsia and gastric adenocarcinoma and lymphoma. The studies have also demonstrated that removal of the organism by anti-microbial therapy is correlated with the resolution of symptoms and cure of diseasesPrinciple of the test: Purified H. pylori antigen is coated on the surface of microwells. Diluted samples serum is added to the wells and the H. pylori IgG- specific antibody if present binds to the antigen. All unbound materials are washed away. Enzyme conjugate is added which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and substrate and chromogen are added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Storage and Stability: 2-8CLimitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. Precision: A study was performed to document typical assay precision with the Biotech H. pylori IgG EIA product. The mean SD and %CV were calculated for Intra- and Inter-Assay in O. D. value (A450).

Secreted.

Nie has the G1M(17) allotypic marker 97-K and the G1M(1) markers 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

EU also differs in the amidation states of residues 155 166 177 195 198 269 and 272 and in the order of residues 268-272.