IgG Antibodies to Helicobacter pylori ELISA Quantitation Kit Manual

IgG Antibodies to Helicobacter pyloriSample Type: Serum# of Samples: 1X96 Assays
Intended Use: The Helicobacter pylori IgM Test Kit is intended for use in evaluating the serologic status to H. pylori. Introduction: Helicobacter pylori is a spiral bacterium cultured from human gastric mucosa discovered by B. J. Marshall in 1982. Studies have indicated that the presence of H. pylori is associated with a variety of gastrointestinal diseases including gastritis duodenal and gastric ulcers non-ulcer dyspepsia and gastric adenocarcinoma and lymphoma. The organism is present in 95-98% of patients with duodenal ulcers and 60-90% of patients with gastric ulcers. All of the testing performed on biopsy samples is subject to errors related to sampling and interference of contaminated bacteria. An ELISA test for the presence of H. pylori specific IgM antibody is the technique of choice for serologic tests because of its accuracy and simplicity. Principle of the test: Purified H. pylori antigen is coated on the surface of microwells. Diluted serum is added to the wells and the H. pylori IgM specific antibody if present binds to the antigen. All unbound materials are washed away. Enzyme conjugate is added which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls. Storage and Stability: 2-8CLimitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test.

Secreted.

Nie has the G1M(17) allotypic marker 97-K and the G1M(1) markers 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

EU also differs in the amidation states of residues 155 166 177 195 198 269 and 272 and in the order of residues 268-272.