Interleukin-1 Beta ELISA

GWB-A5F360

Immunology MarkersSample Type: SerumSensitivity: 0 35 pg/mLDescription: Immunoenzymetric assay for the in vitro quantitative measurement of human Interleukin 1? (IL-1?) in serum and plasma.
Intended Use: Immunoenzymetric assay for the in vitro quantitative measurement of human Interleukin 1& not; (IL-1& not; ) in serum and plasma. Background: A. Biological activitiesHuman interleukin-1 (IL-1) is a key mediator of the host response to various infectious inflammatory and immunologic challenges. Two distinct polypeptides IL-1 and IL-1& szlig; mediate IL-1 biological activities and bind to the same cell surface receptor. Both are initially synthesized as 31-kDA intracellular precursors that are subsequently found as mature proteins of 17 kDA in monocyte supernates. Membrane-bound IL-1 has also been described and may account for a part of IL-1 mediated local effects. The primary sources of IL-1 are blood monocytes and tissue macrophages. Other specialized cells such as T- and B-lymphocytes various epithelial endothelial and some mesenchymal cells can also produce IL-1. IL-1& szlig; is the major form secreted by monocytes and macrophages which are believed to be the main source of circulating (plasma) IL-1. Inhibitions of IL-1 activity have been described in plasma and other biological fluids. IL-1 affects several unrelated tissues and is a main mediator of the \" acute phase\" inflammatory responses characterised by alterations in metabolic endocrinologic and immunologic functions. This cytokine has an essential role in T-cell activation providing one of the necessary signals for IL-2 (T-cell growth factor) production. It is the main mediator of inflammatory processes by acting on the nervous system (fever sleep anorexia) on bone marrow-derived cells (chemotaxis and/or activation of neutrophils monocytes and lymphocytes) and on various tissues (fibroblast proliferation resorption of cartilage and bone matrices glial cell proliferation stimulation of endothelial cell procoagulant activity etc. ). Most of these activities are directly attributable to IL-1& szlig; but others are mediated in collaboration with other cytokines such as IL-6 interferons and tumor necrosis factor. IL-1 stimulates the production or acts synergistically with these cytokines and the final biological activity is thus the result of a network of interactions between these various mediators. Principles of the method: \' s IL-1? -EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of IL-1 . Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human IL-1 - MAb 2 - HRP the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance which is proportional to the IL-1 concentration. A calibration curve is plotted and IL-1 concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range. Storage and Stability: ? Before opening or reconstitution all kits components are stable until the expiry date indicated on the vial label if kept at 2 to 8C. ? Unused strips must be stored at 2-8C in a sealed bag containing a desiccant until expiration date. ? After reconstitution calibrators controls and specimen diluent are stable for 4 days at 2 to 8C. For longer storage periods aliquots should be made and kept at -20C for maximum 2 months. Avoid successive freeze thaw cycles. ? The concentrated Wash Solution is stable at room temperature until expiration date. ? Freshly prepared WorkingWash solution should be used on the same day. ? After its first use the conjugate is stable until expiry date if kept in the original well-closed vial at 2 to 8C. ? The freshly prepared revelation solution is stable before use for maximum 15 minutes at room temperature and must be discarded afterwards. ? Alterations in physical appearance of kit reagents may indicate instability or deterioration.

Produced by activated macrophages IL-1 stimulates thymocyte proliferation by inducing IL-2 release B-cell maturation and proliferation and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response being identified as endogenous pyrogens and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Belongs to the IL-1 family.