in situ Apoptosis Detection Kit

in situ apoptosis detection KitDescription: Apoptosis is a cell regulation system controlled by gene. It is defined as a physiological process for maintaining individual lives to cause death of cells which are dangerous or unnecessary. Apoptosis is much associated with cell growth differentiation and other physiological cellular phenomena especially regarding immune system. It is now manifest that apoptosis plays a significant role in various stages of cancer cells development; growth or proliferation inhibiton disappearance differentiation induced by physical factors (like radiation heat) and by chemical factors (ex. medicine). One of the features of apoptotic cells is fragmentation of chromatin DNA at nucleosome level (185 bp). This fragmentated DNA can be detected histochemically by terminal labeling. TUNEL an acronym for Terminaldeoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling is a effective method for measuring the DNA fragments resulting from the apoptotic activation of intracellular endonucleases. Fluorochromelabeled nucleotides are in situ incorporated onto the ends of these DNA fragments allowing histologic localizaion and individual cells to be detected. # of Samples: 1X96 AssaysPrinciple of the test: TUNEL method uses Terminaldeoxynucleotidyl transferase to label 3\' -OH ends of DNA fragments that are generated during the process of apoptosis. The cells undergoing apoptosis are specifically labeled with fluorescein-dUTP with high sensitivity allowing the immediate detection by viewing with a fluorescence microscope. Since incorporated fluorescence can also be detected with peroxidase-labeled anti-fluorescence antibody it is possible to detect with an light microscope.