TOPBP1 BLOCKING PEPTIDE

GWB-302FD0

TRIS buffered saline

This peptide contains epitopes that are recognized by our anti-TOPBP1 antibody thereby blocking the antibody\' s ability to bind to TOPBP1.

Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase By similarity. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents UV or gamma irradiation. Ref. 5Ref. 6Ref. 7Ref. 8Ref. 9Ref. 10Tissue specificityHighly expressed in heart brain placenta lung and kidney. Ref. 1Induction: Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon. Ref. 9Post-translational modificationPhosphorylated on serine and threonine residues in response to X-ray irradiation. Ref. 6Ref. 7Ref. 13Ref. 14Ref. 15Ref. 16Ref. 18Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. Ref. 7Sequence similaritiesContains 8 BRCT domains. Sequence cautionThe sequence BAA13389. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA34202. 1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.