MeCP2 Antibody

ArtNr	ABC-AP2545d
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MECP2, Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2
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Primary Accession	P51608
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, WB
Bio Background	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Bio References	dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005). Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005). Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004). Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004). Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).
Clonality	Polyclonal
Clone/Animal Names	RB25959
Gene ID	4204
Gene Name	MECP2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-MeCP2(S423) Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: A431 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	All lanes : Anti-MeCP2 Antibody at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: A431 whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	AP2545D staining MeCP2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:500
Legend image 4	AP2545D staining MeCP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/200) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:200
Other Accession	Q95LG8
Isotype	Rabbit IgG
Calculated MW	52441
Antigen Region	400-428
Antigen Source	MACFA
Target/Specificity	This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from human MeCP2.

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