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ABCB5 Antibody (N-term)

ABCB5 Antibody (N-term)

ArtNr	ABC-AP6122a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ABCB5, ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5
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Primary Accession	Q2M3G0
Antigen Type	Synthetic Peptide
Application	FC, WB
Bio Background	ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.
Bio References	Frank, N.Y., Cancer Res. 65 (10), 4320-4333 (2005) Chen, K.G., Pigment Cell Res. 18 (2), 102-112 (2005) Frank, N.Y., J. Biol. Chem. 278 (47), 47156-47165 (2003)
Clonality	Polyclonal
Clone/Animal Names	RB16781
Gene ID	340273
Gene Name	ABCB5 (HGNC:46)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-ABCB5 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: A375 whole cell lysate Lane 5: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	All lanes : Anti-ABCB5 Antibody (N-term) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Western blot analysis of ABCB5 Antibody (N-term) (Cat.#AP6122a) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Overlay histogram showing HepG2 cells stained with AP6122a(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6122a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 5	FC
Dilution image 5	1:25
Legend image 6	Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Type image 6	FC
Dilution image 6	1:10~50
Legend image 7	Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# AP6122a on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver
Type image 7	FC
Dilution image 7	1:10~50
Isotype	Rabbit Ig
Calculated MW	138641
Antigen Region	1-30
Antigen Source	HUMAN
Target/Specificity	This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
NCBI Accession	NP_001157413.1; NP_001157414.1; NP_001157465.1; NP_848654.3

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ABC-AP6122a-datasheet.pdf

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Menge: 400 ul

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