MMP12 Antibody (C-term)

Synthetic Peptide

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.

Polyclonal

4321

Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of MMP12 Antibody (C-term) (Cat. #AP6196a) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

1:10~50

WB

Western blot analysis of anti-hMMP12-R406 Pab (Cat. #AP6196a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. MMP12(arrow) was detected using the purified Pab;

1:1000

WB

Anti-MMP12 Antibody at 1:1000 dilution + human lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

NP_002417

54002

HUMAN

NP_002417.2