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CAV3 Antibody (N-term)

CAV3 Antibody (N-term)

ArtNr	ABC-AP6516A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CAV3, Caveolin-3, M-caveolin
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Primary Accession	P56539
Antigen Type	Synthetic Peptide
Application	WB, IHC-P, FC
Bio Background	CAV3 is a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in its gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD).
Bio References	Garg, V., Biochem. Biophys. Res. Commun. 385 (3), 472-477 (2009) Cai, C., . Biol. Chem. 284 (23), 15894-15902 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB19453
Gene ID	859
Gene Name	CAV3
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-CAV3 Antibody (N-term)at 1:500 dilution + human normal uterus lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:500
Legend image 2	Western blot analysis of CAV3 antibody (N-term) (Cat.# AP6516a) in 293 cell line lysates (35ug/lane). CAV3 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with CAV3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P51638; Q3ZDQ5; P51637; Q2KI43
Predicted	B, M, Pig, Rat
Isotype	Rabbit Ig
Calculated MW	17259
Antigen Region	5-31
Antigen Source	HUMAN
Target/Specificity	This CAV3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-31 amino acids from the N-terminal region of human CAV3.
NCBI Accession	NP_001225.1; NP_203123.1

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ABC-AP6516A-datasheet.pdf

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