IL1A Antibody (Center)

Synthetic Peptide

IL1A is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis.

Polyclonal

3552

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of IL1A Antibody (Center) (Cat. #AP6860c) in HepG2 cell line lysates (35ug/lane). IL1A (arrow) was detected using the purified Pab.

1:1000

WB

Immunohistochemical analysis of paraffin-embedded human esophagus tissue using AP6860c performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

1:500

FC

Overlay histogram showing Jurkat cells stained with AP6860c(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

FC

Rabbit Ig

177-206

This IL1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the Central region of human IL1A.