CBFB Antibody (Center)

Synthetic Peptide

CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.

Polyclonal

865

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of CBFB Antibody (Center) (Cat. #AP6905c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

1:10~50

WB

CBFB Antibody (Center) (Cat. #AP6905c) western blot analysis in K562 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the CBFB antibody detected the CBFB protein (arrow).

1:1000

IHC-P

CBFB Antibody (Center) (Cat. #AP6905c) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

Rabbit Ig

61-90

This CBFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human CBFB.